Dissertation Defense Announcements

Candidate Name: Shreya Goyal
Title: VPS501, A NOVEL SNX-BAR PROTEIN INVOLVED IN AUTOPHAGY
 October 18, 2021  12:00 PM
Location: Zoom
Abstract:

Careful control of intracellular signaling pathways plays an important role in a cell’s ability to maintain stable internal conditions in the face of an ever-changing extracellular environment. This is particularly true as it relates to the process of cellular self-eating or autophagy. Macroautophagy (herein referred to as autophagy) is a catabolic process by which unneeded or damaged cellular components are sequestered as cargo into unique double-membrane vesicles called autophagosomes which fuse to the vacuole (yeast lysosome) to be metabolized. The autophagy-related (Atg) proteins that mediate and regulate the process are evolutionarily conserved across all autophagy pathways, including starvation-induced bulk autophagy and cargo-selective autophagy pathways. The central theme of this thesis is to understand how autophagy is affected by lipids and regulatory proteins in yeast. In this thesis, we have summarized the field’s understanding of lipid homeostasis and trafficking during autophagy and autophagosome formation. Furthermore, we have extended this knowledge by discovering a clear interplay between autophagy and the SNX-BAR protein subfamily. In recent years, the SNX-BARs have been reported to have emerging roles in autophagy, however, such mechanisms of action have been primarily indirect. In this thesis, we have characterized a novel SNX-BAR protein, we have termed Vps501 and have found it directly affects autophagy which brings to light a new role of SNX-BAR proteins in autophagy regulation.



Candidate Name: Meghan Wally
Title: Factors Influencing Clinician Decision Making Regarding Opioid Prescribing and Pain Management
 October 05, 2021  1:00 PM
Location: Zoom Meeting ID: 955 8016 6558 Passcode: 137539
Abstract:

Opioid overdose deaths have increased substantially over the past fifteen years. I characterized the experience of the medical community and measured the multi-level factors influencing opioid prescribing within the context of legislation and clinical decision support interventions.

My content analysis of letters to the editor in JAMA demonstrated that physicians seek to balance pain management and the adverse effects of opioids. Physicians took ownership of their role in the epidemic but called upon the government and community to help address the issue.

My interrupted time series study revealed that legislation resulted in patients with acute musculoskeletal injury (n=12,918) having 17.7% increased frequency (p<0.001) of receiving a perception for <7 days, climbing to 77.1% of all opioid prescriptions. Physician and facility characteristics accounted for 30% and 9% of the observed variation, respectively.

A clinical decision support intervention lowered the percent of patients with chronic musculoskeletal conditions (n=1,290,746) receiving an opioid by 1.6% (p=0.0002) but had no effect on dose. Practice accounted for 24% of the variation in safe opioid prescribing scores.

Collectively, this research presents a sophisticated and nuanced understanding of the multi-level factors which influence guideline-concordant opioid prescribing. These data can inform tailored interventions and guide decision-making and policy.



Candidate Name: John Borek Jr.
Title: Vehicle Velocity Trajectory Optimization in Non-deterministic Environments
 October 04, 2021  12:00 AM
Location: https://uncc.zoom.us/j/99047599972?pwd=ZTJzelBPYXVRVU95NllyMnhrNEthdz09
Abstract:

Optimally modulating a vehicle's speed profile through highway, urban, and suburban environments can result in pronounced fuel savings, particularly with heavy-duty vehicles. However, existing strategies for speed profile optimization traditionally rely on aspirational and often deterministic assumptions, which cease to be accurate in the presence of real-world features such as non-deterministic traffic and actuated signalized intersections. The research in this dissertation establishes a hierarchical Green-Light Approach Speed (h-GLAS) strategy for controlling vehicles traveling through non-deterministic highway, urban, and suburban environments. The h-GLAS strategy utilizes vehicle-to-infrastructure (V2I) communication to receive information about the route's topology, speed limits, and signal phase and timing (SPaT). For suburban environments that employ semi- and fully-actuated signalized intersections, past signal timing information is used forecast the future values of the signal phase lengths. This information is used to construct a desired velocity profile to be tracked by a semi-economic model predictive controller (MPC), which computes the optimal wheel force command for the vehicle. When traveling through highway environments, the desired velocity profile represents a globally optimal dynamic program solution, computed offline before the beginning of a trip. For urban and suburban environments, however, the velocity profile is constructed to allow the vehicle to arrive at upcoming intersections when the probability of a green signal is maximized. The semi-economic MPC minimizes a quadratic objective function, which reflects a trade-off between minimizing mechanical energy expenditure and braking effort, with tracking the supplied desired velocity profile. This MPC is unable to maintain vehicle following constraints without changing its convex nature, so a command governor (CG) is located downstream to maintain vehicle-following constraints efficiently. The CG modifies the MPC's control action by the minimal amount necessary to maintain safe vehicle following. Using the simulation packages from PTV VISSIM, the h-GLAS is validated against real signal timing algorithms within a stochastic traffic environment parameterized by real-world data. Simulation results show that the h-GLAS controller is capable of significantly reducing a vehicle's fuel consumption by 16%-26% when compared to a baseline control strategy traveling through the same suburban environment.



Candidate Name: Vivek F. Pulikkal
Title: Electrochemical Mineralization of Per- and Polyfluoroalkyl Substances
 September 21, 2021  10:00 AM
Location: EPIC 3336
Abstract:

Per- and polyfluoroalkyl substances (PFAS) are fluorinated organic compounds with broad applications in aqueous film-forming foams (AFFF) for firefighting, lubricants, waterproof and stain-resistant products. Perfluorooctanoic acid (PFOA), a legacy PFAS, is toxic and carcinogenic. Currently, PFOA is replaced by the ammonium salt of a perfluoroalkyl ether carboxylic acid known as GenX. Nevertheless, PFOA is expected to be present in the environment for an extended period after its phasing out due to its recalcitrant nature. In addition, GenX is predicted to have similar toxicity as PFOA. Various PFAS, including PFOA and GenX, have been widely detected in surface water and groundwater in the United States and worldwide. The current treatment practice for PFAS fails to provide a permanent solution and is likely to increase the risk of recontamination of surface water and groundwater.
Among various destructive methods, electrochemical mineralization, which uses electric power to transform PFAS into bicarbonate and fluoride, is a promising option. However, past studies on electrochemical mineralization of PFAS have limitations such as low PFAS mineralization and incomplete fluorine mass balance. Hence, this study proposes a sequential treatment with ion exchange to capture and concentrate PFAS in surface water and groundwater into a low volume of brine, followed by electrochemical treatment of the PFAS-containing brine. This study will focus on the electrochemical oxidation part of the treatment train and examine the treatment performance using PFOA, GenX, and AFFF waste streams as examples.



Candidate Name: Chuqin Li
Title: Leveraging social media to better understand people's obesity related behaviors
 September 21, 2021  8:30 AM
Location: Zoom: https://uncc.zoom.us/j/93433321104?pwd=QkU5UFhJQ2xkOFVrTllISm1kSk15QT09 Meeting ID: 934 3332 1104 Passcode: 542804
Abstract:

With the worldwide prevalence of obesity doubling over the past 30 years across a wide range of demographic and socioeconomic groups, the obesity epidemic is a major public health challenge today. Excessive food consumption and lack of exercise are two major contributors to obesity. In recent years, these two activities have been integrated into our daily lives together with social media. A growing body of literature delineated how social networks impact people's health-related behaviors and suggested social media has a role in affect people's obesity-related behaviors. This study aims to gain an insightful understanding of which online social factors are impacting users' obesogenic behaviors and explore computational methods to examine those behaviors using social media data.

Our work consists of three overall research aims. In the first aim, a systematic review was conducted to examine online social factors concerning obesity. A total of 1,608 studies that related to obesity and social media were collected from the three most popular electronic databases for the field. After close inspection, 50 studies were further examined and ten types of online social factors were identified within four-level social-ecological model, which was used to explain each factors' potential impact on an individual from varying levels of online social structure to user's connection to the real world. In the second aim, we learned how the local food environment influences state level obesity rate using social media data. Publicly available Yelp and MyFitnessPal data were collected via a novel approach to characterize the local food environment. A statistically significant correlation between the state's food environment and state obesity rate was observed. We further built a computational model to predict the state-level obesity rate using aforementioned data, in which we achieved a Pearson correlation of 0.791 across US states and the District of Columbia. In the third aim, we studied how a major social disrupting event, COVID-19 shutdown, affects users' dietary behavior using social media data. Tweets relating to people's dietary behavior with images from April to June of 2019, 2020, and 2021 were collected. An observational study of behavior patterns was conducted by using image classification models, visualization tools, and text analysis methods. People are found eating more healthier food during complete and partial shutdowns than before Covid-19. Results of this dissertation could help the public health agencies, policymakers, organizations and health researchers to better utilize social media to carry out obesity-related education, obesity surveillance, and develop public health policy to address this challenge.



Candidate Name: Armin Amirazar
Title: Evidence-Based Human-Centric Lighting Assist Tool towards a Healthier Lit Environment
 September 17, 2021  3:30 PM
Location: Remotely
Abstract:

Light is an essential element of building design that influences human health, comfort, performance, and well-being. Humans' daily rhythms in behavior and physiology, such as wake/sleep patterns, have evolved under natural light-dark cycles over millions of years. Nowadays, as we spend a large proportion of our time in the built environment, we are exposed to less light during daytime hours and more light during nighttime hours than what we would have naturally received across day and night. Thus, inappropriate and insufficient personal light exposure during the day and night can negatively affect this standard rhythm and is associated with a range of psychological, physical, and mental health issues. While most lighting design recommendations and standards have been limited to addressing the energy and visual aspects of light, this trend has been criticized, and current standards acknowledge the link between light and human health. Moreover, lack of low-cost and reliable tool to track and monitor the characteristics of light exposure as a stimulus that affects the human circadian system is evident.

This dissertation proposed a novel user-centric lighting assist tool consisting of a low-cost and wearable spectrometer to measure light spectrum and an interactive dashboard to visualize the collected data in meaningful and easy to understand quantities. Three studies covering the proposed tool are presented to 1) develop a low-cost and wearable spectrometer using Artificial Neural Networks (ANNs); 2) examine practical applicability of wearable spectrometer in the real-world environment ; and 3) develop and test the usability of an interactive dashboard for continuous tracking of personal lighting conditions. The first study examines the performance, accuracy, and fabrication challenges of developing a low-cost, wearable and wireless spectrometer to measure Spectral Power Distributions (SPD) of light sources using ANNs. Neural network was identified as an effective method for improving the accuracy of the developed spectrometer. Additionally, the developed spectrometer offers real-time communication that enables it to be integrated into IoT-based intelligent lighting systems for tailoring indoor lighting systems according to individual circadian needs. The second study examines the practical applicability of developed spectrometer to continuously record personal lighting conditions of office workers in real-world environment. The study provides insights for enhancing occupants health and well-being within the built environment. The third study examines the potential of a web-based app to enable healthier living with light. By engaging the end-user directly throughout the entire process of design and development of the interactive dashboard, the study identified the interactive dashboard as a useful and usable tool for end-users.

This dissertation is one of the first attempts to develop a low-cost and wearable spectrometer together with an interactive application to provide vital information regarding the non-visual effects of light on health by real-time tracking of personal lighting conditions. The findings of this dissertation demonstrates the importance of an affordable and accessible human-centric lighting assist tool as a powerful driver of promoting healthy behavior change in buildings, outlining new directions in the design of buildings that are not only comfortable and energy efficient, but also healthier for their occupants.



Candidate Name: Alicia D. Dervin
Title: Exploring Diasporic Dialects for Black Women in College Composition and Communication: A Critical Reflective Narrative Inquiry
 September 17, 2021  11:00 AM
Location: Virtual (Zoom)
Abstract:

Historically, standard English language ideologies have been perpetually ingrained in American educational practices and policies (Smitherman, 2017; Wong & Teuben-Rowe, 1997). These practices are not limited to K-12 studies and maintain a position of dominance in higher education (Álvarez-Mosquera & Marín-Gutiérrez, 2020). Calls for diversity in curriculum and pedagogical practices currently involve increasing demands for linguistic inclusion that reflects the diversity of student populations (CCCC, 2020). This study explores how Black women students across the diaspora who use home and/or native languages, dialects, and accents navigate their identities in academic spaces of higher learning where standard English language ideologies are often the only acceptable language varieties that are valued or encouraged. Data was collected through the use of virtual semi-structured interviews using Seidman’s (2006) method of three 90-minute interviews. A single focus group interview with all three participants was conducted as well. All interviews took place during the summer of 2021. Data was analyzed using a qualitative, narrative-based approach that included emphasis on both small stories (De Fina and Georgakopoulou, 2012) and dominant narratives (Lyotard, 1984). This research suggests the experiences of Black women in college composition and communications include themes like feelings of rejection, inadequacies, pressure to conform, and a lack of linguistic agency.



Candidate Name: Sagar Satyanarayana
Title: TOWARDS ENDING THE HIV EPIDEMIC ONE COUNTY AT A TIME AN EVALUATION FRAMEWORK FOR SUBCOUNTY-LEVEL USAGE OF PRE-EXPOSURE PROPHYLAXIS FOR EXPOSING DISPARITIES AND GUIDING TARGETED INTERVENTIONS
 September 15, 2021  12:00 PM
Location: Please email ssatyana@uncc.edu for the ZOOM link
Abstract:

About 1.1 million Americans were living with HIV in 2019, with the total lifetime cost to treat a single person with an HIV infection estimated to be around $501,000. PrEP, a pill taken daily by mouth, contains antiretroviral drugs and is highly effective in preventing the acquisition of HIV. Even though PrEP coverage in the US improved from 9% in 2016 to 18% in 2018, considerable disparities in PrEP prescriptions exist in different geographic, racial/ethnic, and age groups. Existing metrics to measure PrEP overestimates PrEP coverage. Moreover, previous studies conducted at the national and state levels often fail to capture disparities in PrEP use within the county and cannot be used by county public health officials to conduct targeted interventions. This dissertation establishes an evaluation framework for HIV prevention by measuring PrEP usage at subcounty levels.
The PrEP coverage increased annually throughout the study period in the county. The G2ZMC PHI had a significant gradual effect of about nine PrEP patients every month. The results of this dissertation quantified and identified opportunities for improvement in PrEP use in underserved groups. This work can be extended to other counties and provide a foundation for similar studies on other emerging infectious diseases.



Candidate Name: Paula Loman-Cortes
Title: Design, Synthesis, Characterization and Simulation of Polyhedral Oligomeric Silsesquioxane Porphyrin Molecules to Fabricate Self-Assembled Nanoparticles And Their Use For Photodynamic Therapy
 September 14, 2021  2:00 PM
Location: https://uncc.zoom.us/j/92476921781?pwd=Q2pIMDRQc1ZiUDMrSlhOYXpnNmpiQT09
Abstract:

Supramolecular aggregates can have specific nanostructures that give them a variety of functionalities, making them useful for many applications in energy, catalysis, medicine, biotechnology and other scientific fields. These functional nanostructures are built via the self-assembly of building blocks with particular physicochemical properties. Different molecular interactions participate in the self-assembly processes such as metallic, ionic, van der Waals forces, electrostatic, hydrophobic, H-bonding, and π- π stacking. Therefore, the building blocks for self-assembly are molecules that are pre-designed to supply these interactions in a given environment; in addition, they can provide a desired functionality.
Polyhedral oligomeric silsesquioxane (POSS) is a promising scaffold to be used as delivery system. When covalently linked to a photosensitizer, POSS has an influence on the self-assembly behavior of the photosensitizer, modifying its properties, potentially enhancing its efficacy toward photodynamic therapy (PDT).
In this Thesis, I describe my work on the study of polyhedral oligomeric silsesquioxanes (POSS) porphyrin derivatives as building blocks, their self-assembly and application in photodynamic therapy (PDT). We envision that the chemical tunability of POSSs can be used as a promising option to improve the delivery and performance of photosensitizers.



Candidate Name: Alicia W. Davis
Title: Concrete Roses: An Exploration of the Paralleled Lived Experiences of Black Girls and Women in K-12 Schools
 September 07, 2021  9:00 AM
Location: Zoom
Abstract:

The schooling and leadership experiences of Black girls and women are overlooked as they are often intertwined with the experiences of Black males or White women. Historically, the academic, mentoring, leadership, and mental health needs of Black girls and women have been neglected and challenged in educational settings. The purpose of this qualitative study was to explore the racialized lived experiences of Black girls and women in K-12 schools from a student and Black school administrator perspective. The findings from this dissertation indicate the following: a) Black girls and women lack support and nurturing at all levels in education (student, AP, and principal); b) Black girls and women need spaces in schools to feel supported, collaborate and create actions and policies on how to best meet their needs; c) the mental health of Black girls and women must be considered sacred and protected at all times; and d) Black girls and women need to be included in decision making processes at all school levels.